Determination of the Levels of Aflatoxin in Peanut Butter using the Aflaprep Immunoaffinity Column Clean-Up Procedure: Collaborative Trial

Twenty one United Kingdom laboratories participated in a collabora- tive trial to evaluate the Aflaprep imt?runodlrtniry column .for the de- termination of aflatoxins in peanut butter. Ed.ch pdrticipant received ten randomly numbered samples ofroasted peanut butter which were prepared as fiye sets of blind duplicates. One set of samples was blank, uncontaminated peanut butter. The other four sets oJ samples contained dilferent concentrations oJ total aJlatoxin – ca 8; 12; 35; 5 0pg/kg. These latter samples were prepared.from naturally contami- nated peanuts, the mdjor contamination being from aflatoxin G I Par- ticipdting laboratories were instructed to Jbllow a set procedure with regard to the use of the immunoaftinity column for extraction and r)ere to quantitate total aflatoxin levels by HPLC with post-column deriyitisation with iodine. The relative :ttdndard deviations for repro- ducibiliE- @SD) for the total dllatoxin levels, from the f en) one Iaboratories, for the firu’ concentrations of contaminated pednut butter, were respectively; 54% (on a mean of 7.0p9/kg); 30?6 (on a mean of 1l.3pg/kg); j5% (tn a mean oJ 3j.2pg/kg); and j6’% (on a mean of 47.7pg/kg).

Fiona M. Worner, Alan L. Patey and Roger Wood. Determination of the Levels of Aflatoxin in Peanut Butter using the Aflaprep Immunoaffinity Column Clean-Up Procedure: Collaborative Trial. J. Assoc. Publ. Analysts., 1992, 28, 1-10.