Validation of a Method for the Determination of Theobromine in Feed Materials and Compound Feeds by Liquid Chromatography with UV Detection or with Tandem Mass Spectrometry Kirstin M Gray a and D Thorburn Burnsb a
Abstract
An inter-laboratory validated HPLC separation coupled with UV detection method for the determination of theobromine in animal feed materials and in compounded feeds is described using 7-(β-hydroxyethyl) theophylline as the internal standard. If more forensically robust identification of the presence of theobromine is required, the same HPLC separation can be coupled to a tandem mass spectrometer and theobromine-d3 used as the internal standard. At the time of publishing, the method described in this paper is in the process of a formal vote before being published as a CEN standard.
